DNA

Part:BBa_K156003

Designed by: George McArthur IV   Group: iGEM08_Virginia   (2008-10-27)


BioBrick placeholder 3 (Prefix - ApoI (NotI) NsiI - Suffix)

This BioBrick Placeholder part is one of a set of eight that represent a new technical standard useful in composite part construction. Flanked by the standard BBa restriction sites, these parts are made of internal restriction sites that are unique but compatible with the standard sites (see below). This allows insertion of a desired part (e.g., RBSs, coding regions) within a previously-made construct.

Compatible sites:

EcoRI - ApoI & MfeI
XbaI & SpeI - AvrII, NheI
PstI - NsiI, SbfI

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 43
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 44
    Illegal PstI site found at 58
    Illegal NotI site found at 7
    Illegal NotI site found at 29
    Illegal NotI site found at 51
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 44
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 44
    Illegal PstI site found at 58
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None